Synthetic Plasmid DNA Injection Challenge

Dive into the world of synthetic biology with this intriguing puzzle that involves the design of a synthetic plasmid and its injection into a bacterial cell. Your task is to strategically map out the plasmid DNA sequence to ensure successful expression of desired proteins. Consider genetic promoters, markers, and payload genes, keeping in mind the plasmid's stability and functionality within the host. The goal is to optimize the injection process for maximum efficiency and minimal cell disruption. Unravel the complexities of genetic engineering through this immersive puzzle!